Navegando por Autor "Almeida, Leonardo Augusto de"

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The role of IL‐10 in immune responses against Pseudomonas aeruginosa during acute lung infection.
(2020) Belo, Valéria Aparecida; Pereira, Jéssica Assis; Souza, Sara Franchin Duarte de; Tana, Fernanda de Lima; Pereira, Bruna Pinheiro; Lopes, Débora de Oliveira; Ceron, Carla Speroni; Novaes, Rômulo Dias; Corsetti, Patrícia Paiva; Almeida, Leonardo Augusto de
Pseudomonas aeruginosa is considered an opportunistic pathogen of great clinical importance. The clearance of this bac- terium occurs through recognition of the pathogen by innate immune system receptors, leading to a lung infammatory response. However, this response must be controlled via immunoregulatory pathways. In this study, we evaluate the role of endogenous murine IL-10 after acute infection with the virulent strain P. aeruginosa PA14. To assess the role of IL-10, intratracheal infection with the PA14 strain was performed in C57BL/6 or IL-10 KO mice. The PA14 strain was recovered in both types of animals, although IL-10 KO mice presented a higher number of viable bacteria in the lung when compared to the C57BL/6 group. Histopathological and stereological analyses showed that IL-10 KO mice had higher tissue damage and infammatory infltrate when compared to control animals. The activity of MMP-9 but not MMP-2, as well as IL-6 and TNF-α expression, were augmented in the lungs of infected animals and was much more evident in IL-10 KO animals when compared to the other analyzed groups. This work indicates that endogenous IL-10 control P. aeruginosa infection, the expression of pro-infammatory genes, MMP-9 activity and histopathological processes of the infectious process in question.